| 2026 The National Pastime Players | ||
| National Conference | ||
| Atlantic Division | ||
| Team | Manager | |
| LAD | Lady Lake Live Wires | Cheryl Balas |
| VTM | Vermont Lake Monsters | Nate Bishop |
| WOR | Worcester Red Sox | Stephen DiCaprio |
| POR | Portland Sea Dogs | Stephen Miller |
| BAT | Batavia Muckdogs | Pete Gath |
| ABI | Aberdeen IronBirds | Steve Ruth |
| STM | Southern Tier Spicy Meatballs | Jedd Gardner |
| Great Lakes Division | ||
| Team | Manager | |
| DAY | Dayton Dragons | Jamie Dyke |
| LHV | Lehigh Valley Iron Pigs | Ray Perrotta |
| ERI | Erie Seawolves | Scott Polohonki |
| LAN | Lansing Lugnuts | Curt Manning |
| KAN | Kane County Cougers | Jay Smogolski |
| SCF | Schaumburg Flyers | Jerry Miller |
| American Conference | ||
| South Division | ||
| Team | Manager | |
| GWN | Gwinnett Stripers | Greg Miller |
| JAX | Jacksonville Jumbo Shrimp | Scott Schmitt |
| CAR | Carolina Mudcats | Kevin Sullivan |
| BGH | Bowling Green Hot Rods | Will Riney |
| DUR | Durham Bulls | Myra Cochran |
| FLR | Florence Freedom | Darrell Cox |
| SOU | South Bend Cubs | Shane Burton |
| Frontier Division | ||
| Team | Manager | |
| QCR | Quad City River Bandits | John DiGiovanni |
| OMA | Omaha Storm Chasers | Nick Hagen |
| FTW | Fort Wayne TinCaps | Chris Webb |
| ABQ | Albuquerque Isotopes | Zach Kruep |
| SFE | Santa Fe Fuego | Enda Mulcahy |
| SAC | Sacramento River Cats | James McDaniels |